Enhancement of anaerobic digestion of phoenix tree leaf by mild alkali pretreatment: Optimization by Taguchi orthogonal design and semi-continuous operation

This study aimed at evaluating the valorization of a typical yard waste, phoenix tree leaf (PTL), through mild alkali pretreatment followed by anaerobic digestion (AD). To this end, L9 Taguchi orthogonal biochemical methane potential (BMP) tests and semi-continuous AD experiments were conducted to examine the optimum pretreatment condition and the long term effect of alkali pretreatment on AD. The community structure evo-lutions were analyzed by high throughput 16S rRNA gene pyrosequencing. The results indicated that alkali pretreatment was effective on decrystallization and releasing more surface of PTL for enzyme attacking. The methane yield was positively correlated with lignin removal (R-2=0.8242). In semi-continuous mode, 151.5 +/- 7.9 mL/g VS of the methane yield was obtained for alkali pretreated PTL, which was 80% higher than that of untreated one. Microbial community analysis indicated that alkali pretreatment led to a higher abun-dance of dominated bacteria (Bacteroidetes and Clostridia) and archaea of Methanosaeta.