Journal
BIORESOURCE TECHNOLOGY
Volume 313, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.123666
Keywords
Glycerol; Pretreatment; Rhodosporidium toruloides; Lipid; Carotenoid
Funding
- Sugar Research Australia (SRA)
- Australian Government Department of Agriculture, Water and the Environment through the Rural R&D for Profit Program
- Queensland Government Department of Agriculture and Fisheries
- Cotton Research and Development Corporation
- Forest & Wood Products Australia
- Australian Pork Ltd
- Southern Oil Refining
- Queensland University of Technology
- NSW Department of Primary Industries
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Two-step dilute acid and acid-catalysed glycerol pretreatment was developed to maximise sugar yield from sugarcane bagasse. At the laboratory scale, dilute acid pretreatment at 130 degrees C followed by acid-catalysed glycerol pretreatment at 170 degrees C led to a total sugar (C5 + C6) yield of 82%, 31% higher than that from one-step acid-catalysed glycerol pretreatment. At the pilot scale, the two-step dilute acid and acid-catalysed glycerol pretreatment led to a maximum sugar yield of 74%, 13% higher than that from one-step pretreatment with 52% reduction in glycerol usage. The enzymatic hydrolysate containing glucose and residual glycerol were used to produce microbial oils by a Rhodosporidium toruloides strain. A fed-batch cultivation strategy led to the pro-duction of 44.8 g/L cell mass, including 26.6 g/L oil, 8.6 g/L protein and 12.7 mg/L carotenoid. The cell mass and oil yields were 19% higher than those from batch cultivation as feedstock inhibition and catabolite re-pression were alleviated.
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