Green biomimetic silver nanoparticles utilizing the red algaeAmphiroa rigidaand its potent antibacterial, cytotoxicity and larvicidal efficiency

The present investigation reports a simple, rapid, inexpensive, and eco-friendly approach for synthesizingAmphiroa rigida-mediated silver nanoparticles (AR-AgNPs) for the first time. The biomimetic synthesized AR-AgNPs were characterized by both spectral and microscopic analysis. The UV-visible spectrum showed the surface plasmon peak at 420 nm, which indicated the formation of AR-AgNPs. X-ray diffraction characterization of AR-AgNPs showed a face-centered cubic crystal (25 nm) and the transmission electron microscope micrograph showed spherical shape. The functional group of polysaccharide that plays a major role as a stabilizing and reducing agent is confirmed by Fourier-transform infrared spectroscopy. The biomimetic synthesized AR-AgNPs showed antibacterial activity againstStaphylococcus aureus(21 +/- 0.2 mm) andPseudomonas aeruginosa(15 +/- 0.2 mm).Further, the cytotoxic effects of AR-AgNPs against MCF-7 human breast cancer cells were observed through acridine orange-ethidium bromide and Hoechst staining. Besides, AR-AgNPs are found to be inhibit the growth of 3rd and 4th instar larvae ofAedes aegyptiin a dose-dependent manner.

Automated summary

The study reported a biomimetic approach to synthesize AR-AgNPs, which were characterized using various techniques and found to possess antibacterial and cytotoxic effects.