Biotransformation of two citrus flavanones by lactic acid bacteria in chemical defined medium

Microbial processes are being developed to transform flavonoid glycosides to varieties of metabolites with higher bioavailability. The aim of this study was to determine the metabolic activity and survival of five lactic acid bacteria (LAB) stains (L. rhamnosusLRa05,L. caseiLC89,L. plantarumN13,L. acidophilusLA85, andL. brevisLB01) in two different citrus flavanone standards (hesperetin-7-O-rutinoside and naringenin-7-O-rutinoside). The enzymatic activity, metabolites, antioxidant activities, and alpha-glucosidase inhibition property in the two standards were also investigated before and after incubated with LAB. The medium contained standards permitted survival of the five LAB stains. All strains exhibited beta-glucosidase activity. Of the five LAB strains tested, justL. plantarumN13 andL. brevisLB01 have the ability to metabolize hesperetin-7-O-rutinoside, onlyL. plantarumN13,L. acidophilusLA85, andL. brevisLB01 could metabolize naringenin-7-O-rutinoside, moreover,L. acidophilusLA85l was the strain with the highest biotransformation ratio of naringenin-7-O-rutinoside.L. acidophilusLA85 andL. plantarumN13 can degrade naringenin-7-O-rutinoside into naringenin.L. brevisLB01 can degrade hesperetin-7-O-rutinoside into hesperetin, 3-(4 '-hydroxyphenyl)-2-propenoic acid, 3-(3 '-hydroxy-4 '-methoxyphenyl)hydracrylic acid, and 3-(4 '-hydroxyphenyl)propionic acid. Incubation ofL. acidophilusLA85 in naringenin-7-O-rutinoside solution supposed no apparent influence in the biological activities that tested.L. acidophilusLA85 may potentially contribute to the bioavailability of citrus flavanones, and to be applied as functional cultures to obtain more bioavailable and bioactive metabolites in food products or in the human gastrointestinal tract.

Automated summary

This study investigated the metabolic activity and survival of five LAB strains in citrus flavanone standards, revealing that only L. plantarum N13 and L. brevis LB01 have the ability to metabolize hesperetin-7-O-rutinoside, with L. acidophilus LA85 showing the highest biotransformation ratio of naringenin-7-O-rutinoside. The findings suggest that L. acidophilus LA85 and L. plantarum N13 could potentially contribute to the bioavailability of citrus flavanones and be used as functional cultures to enhance the bioactivity of food products.