Journal
BIOCONJUGATE CHEMISTRY
Volume 31, Issue 10, Pages 2283-2287Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.0c00422
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Funding
- ECHO grant from the Dutch Organization for Scientific research (NWO) [711.017.004]
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Catalytic nanostructures have the potency to mimic enzymatic features. In this paper, we show that the complex between hemin and G-quadruplex DNA efficiently catalyzes the modification of proteins with N-methyl luminol derivatives. Final conversions are reached within 15-30 min, and LC-MS analysis of tryptic digests of the proteins shows that the reaction proceeds with chemoselectivity for electron-rich aromatic residues (Tyr >> Trp), and the site-specificity of the modification depends on the sequence and secondary structure folding of the G-quadruplex nanostructure. Furthermore, the modification can be applied on proteins with different biomedical functions, and the nanostructure can be designed to contain a regulatory element in order to regulate protein modification by an external stimulus.
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