Journal
APPLIED OPTICS
Volume 60, Issue 4, Pages A215-A221Publisher
OPTICAL SOC AMER
DOI: 10.1364/AO.404297
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- Universidad Nacional de Colombia [Hermes 50210]
- Universidad EAFIT
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A numerical method is presented in this study to enhance the contrast of intensity hologram reconstructions in DLHM through mathematical operations and parameter adjustments, showing improved reconstruction results.
In this work, a numerical method to enhance the contrast of intensity hologram reconstructions of digital lensless holographic microscopy (DLHM) is presented. The method manipulates the in-line hologram and reference images through mathematical operations between them; additionally, a sharpening operation, functionalized in terms of the parameters of the recording setup, is applied to the said images. The preprocessing of the recorded images produces a modified in-line hologram and a reference wave image from which an intensity reconstruction with a 25% improvement of its contrast, with respect to the conventional reconstruction procedure, is achieved. The method is illustrated with intensity reconstructions of a hologram of a monolayer of polystyrene spheres 1.09 mu m in diameter. Finally, the preprocessing method is validated with a modeled hologram, successfully applied to holograms of the section of the head a Drosophila melanogaster fly and its results are contrasted with those obtained via bright-field microscopy. (C) 2020 Optical Society of America
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