4.4 Article

FAM60A promotes cisplatin resistance in lung cancer cells by activating SKP2 expression

Journal

ANTI-CANCER DRUGS
Volume 31, Issue 8, Pages 776-784

Publisher

LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/CAD.0000000000000952

Keywords

cisplatin resistance; family with sequence similarity 60A; lung cancer; SKP2

Funding

  1. Hangzhou Health Science and Technology Project [20180533B63]
  2. Zhejiang Health Science and Technology Project [2018KY595]
  3. National Natural Science Foundation of China [81860306]
  4. Yunnan Engineering Technology Center of Digestive Disease [2018DH006]
  5. Health Department of Yunnan province [2018NS0083]
  6. Yunling Scholar [YLXL20170002]

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Cisplatin is a widely used chemotherapeutic drug in lung cancer treatment. Most cancer patients eventually develop cisplatin resistance, resulting in a poor prognosis. Previously, we identified a novel marker, family with sequence similarity 60A (FAM60A), that was responsible for resistance in cisplatin-resistant human lung adenocarcinoma A549 (A549/DDP) cells. Here, we investigated the biological effects of FAM60A in A549/DDP cells and explored the underlying molecular mechanisms to understand its functional role in cisplatin resistance. Real-time quantitative PCR and western blot analysis were used to determine the expression levels of FAM60A in A549/DDP cells. FAM60A and SKP2 were knockdown with small-interfering RNA (siRNA). Cancer cell viability was analyzed with flow cytometry. The mRNA and protein expression levels of FAM60A increased significantly and dose-dependently in A549/DDP cells following cisplatin treatment. FAM60A overexpression up-regulated MDR1 expression, inhibited caspase 3, cleaved-caspase 3, and caspase 8 expression, and prevented cancer cell death. Microarray analysis of cells transfected with siRNA against the FAM60A transcript and control samples showed that SKP2 expression was positively regulated by FAM60A. SKP2 knockdown using a short-hairpin RNA reversed the functions induced by FAM60A. These results suggest that overexpression of FAM60A in A549/DDP cells led to SKP2 upregulation and enhanced cisplatin resistance in cancer cells. These provide new insights into chemoresistance and may contribute to reversing cisplatin resistance during lung cancer treatment.

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