Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 59, Issue 52, Pages 23564-23568Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202011198
Keywords
aptamer; cancer cell isolation; cancer diagnosis; live cell membranes
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Funding
- National Natural Science Foundation of China [21974087]
- Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support [20181709]
- Shanghai Rising-Star Program [20QA1405800]
- Innovative Research Team of High-Level Local Universities in Shanghai
- Institute of Molecular Medicine of Shanghai Jiao Tong University
- Recruitment Program of Global Youth Experts of China
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A single-step method for isolation of specific cells based on multiple surface markers will have unique advantages because of its scalability, efficacy, and mildness. Herein, we developed multi-aptamer-mediated proximity ligation method on live cell membranes that leverages a multi-receptor co-recognition design for enhanced specificity, as well as a robust in situ signal amplification design for improved sensitivity of cell isolation. We demonstrated the promising efficacy of our method on differentiating tumor cell subtypes in both cell mixtures and clinical samples. Owing to its simple and fast operation with excellent cell isolation sensitivity and accuracy, this approach will have broad applications in biological science, biomedical engineering, and personalized medicine.
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