Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 59, Issue 50, Pages 22414-22418Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202009240
Keywords
aptamers; gene expression; synthetic biology
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Funding
- European Union ERC [615381]
- German Research Council [MA3442/5-1, MA3442/5-2, SFB1089-P1]
- Projekt DEAL
- European Research Council (ERC) [615381] Funding Source: European Research Council (ERC)
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The investigation and manipulation of cellular processes with subcellular resolution requires non-invasive tools with spatiotemporal precision and reversibility. Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression. This platform significantly reduces the coding space required for genetic manipulation and provides a strong on-switch with almost no residual activity in the dark. It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
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