Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 5, Pages 2637-2642Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202011903
Keywords
cancer; enzyme-activated nanoprobes; fluorescence; neuroblastoma; second near-infrared window
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Funding
- National Key Research and Development Program of China [2016YFA0101503, 2017YFA0205503]
- National Natural Science Foundation of China [21671198, 21703282, 21934007, 81971685]
- Strategic Priority Research Program of Chinese Academy of Science [XDB36000000]
- Natural Science Foundation of Jiangsu Province [BK20170066]
- Youth Innovation Promotion Association of Chinese Academy of Sciences (CAS)
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The novel nanoprobes A&MMP@Ag2S-AF7P can achieve rapid activation in neuroblastoma by inhibiting fluorescence resonance energy transfer between Ag2S QDs and A1094 mediated by MMP14 overexpression, providing a superior tumor-to-normal tissue ratio, instant illumination of the lesion, and well-defined tumor margins, making them a suitable rapid diagnostic reagent for cancer surgical or tissue biopsy procedures.
Accurate intraoperative tissue identification is critical to tumor surgery. However, conventional methods are labor- and time-intensive, which greatly delay the intraoperative decision-making. Herein, a matrix metalloproteinase (MMP)14-activated NIR-II nanoprobe (A&MMP@Ag2S-AF7P) is presented for rapid unperturbed-tissue analysis for ex vivo and in vivo neuroblastoma diagnosis. A&MMP@Ag2S-AF7P displays negligible fluorescence in normal tissues but is activated quickly by inhibiting the fluorescence resonance energy transfer (FRET) between Ag2S QDs and A1094 mediated by MMP14 overexpressed in neuroblastoma; meanwhile, the exposure of the membrane penetrating peptide R9 (TAT-peptide) results in efficient internalization of nanoprobes in the cancer cells, providing superior tumor-to-normal (T/N) tissue ratio. Instant illumination of the lesion and well-defined tumor margins make the nanoprobes a suitable rapid diagnostic reagent for cancer surgical or tissue biopsy procedures.
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