Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 8, Pages 3934-3939Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202010703
Keywords
amyloids; genetic code expansion; proteoforms; proteolytic processing
Categories
Funding
- Wellcome Trust [217249/ Z/19/Z]
- National Research Council of Thailand [MRG6280177]
- Thailand Science Research and Innovation (Global Partnership Program)
- NIH [R01GM122984]
- Yale University West Campus start-up funds
- VISTEC
- Wellcome Trust [217249/Z/19/Z] Funding Source: Wellcome Trust
Ask authors/readers for more resources
A new labeling strategy has been developed in this study for proteolytic proteoforms, showcasing its utility in detecting amyloid-beta peptides.
Polypeptides generated from proteolytic processing of protein precursors, or proteolytic proteoforms, play an important role in diverse biological functions and diseases. However, their often-small size and intricate post-translational biogenesis preclude the use of simple genetic tagging in their cellular studies. Herein, we develop a labeling strategy for this class of proteoforms, based on residue-specific genetic code expansion labeling with a molecular beacon design. We demonstrate the utility of such a design by creating a molecular beacon reporter to detect amyloid-beta peptides, known to be involved in the pathogenesis of Alzheimer's disease, as they are produced from amyloid precursor protein (APP) along the endocytic pathway of living cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available