Biochar amendment improves shoot biomass of tomato seedlings and sustains water relations and leaf gas exchange rates under different irrigation and nitrogen regimes

Biochar amendment has many benefits for improving soil water holding capacity and plant growth particularly under reduced irrigation regimes, yet the underlying biochemical and physiological mechanisms remain largely elusive. The combined effects of biochar addition and deficit irrigation under two N fertilizer regimes on tomato plant growth and physiology were investigated. The results showed that, despite a negative effect on leaf N content, biochar amendment improved the plant water status and leaf gas exchange rates under deficit irrigation, thereby enhanced the biomass (DM) of tomato plants irrespective of N regimes. Even though biochar amendment tended to reduce instantaneous water use efficiency, it significantly enhanced plant water use efficiency (WUEp). Biochar increased soil pH and resulted in an increase in xylem pH, which however did not amplify the root-to-shoot ABA signalling inducing early stomatal closure during deficit irrigation. The principal component analysis (PCA) plot showed that plant water use (PWU) rather than WUEp contributed significantly to the enhanced DM under biochar amendment. Thus, the greater stomatal conductance and transpiration rate and consequently the higher PWU as exemplified by the lowered leaf delta C-13 and delta O-18 values might have contributed to the increased DM of the biochar treated tomato plants. It is concluded that biochar amendment could be a promising practice to enhance tomato seedling growth under reduced irrigation and N fertilization regimes.

Automated summary

Biochar amendment has benefits for improving soil water holding capacity and plant growth under reduced irrigation and N fertilizer regimes. Despite reducing leaf N content, biochar amendment enhances plant water status, gas exchange rates, and water use efficiency under deficit irrigation, leading to increased biomass of tomato plants. The increase in soil pH by biochar does not amplify ABA signaling inducing early stomatal closure, but rather contributes to the enhanced plant water use and growth.