Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 30, Issue 46, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.202003480
Keywords
assembly; DNA; microfluidics; polymersomes; synthetic biology
Categories
Funding
- Federal Ministry of Education and Research of Germany [13XP5073A]
- MaxSynBio Consortium - German Federal Ministry of Education and Research
- MaxSynBio Consortium - Max Planck Society
- German Research Foundation [SFB 1129]
- Volkswagenstiftung
- European Union Horizon 2020 research and innovation program under the Marie Skodowska-Curie grant [792270]
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy via the Excellence Cluster 3D Matter Made to Order [EXC-2082/1-390761711]
- Projekt DEAL
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Polymersomes exhibit increased stability and reduced permeability compared to conventional lipid-based compartments-making them an increasingly popular choice for the bottom-up construction of synthetic cells. Here, the contraction and expansion of functionalized polymersome networks controlled by cholesterol-tagged deoxyribonucleic acid (DNA) is demonstrated. Different types of block-copolymer membranes are systematically screened to design a general framework for the self-assembly of cholesterol-tagged DNA into the polymersome membrane. Since the developed approach is DNA-based, the individual symmetric and asymmetric functionalization of the inner and outer polymersome leaflets is possible, a feature which makes it unique in terms of anchoring flexibility and efficiency. In addition to the variety with regard to functionalization, the choice of DNA and temperature can also be used to manipulate the polymersome contact line and the polymersome bending energy. Overall, the strategy potentially allows for the unprecedented possibility to precisely control the contraction and expansion of polymersome network assembly.
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