Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 30, Issue 49, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.202005531
Keywords
aerogels; bone regeneration; electrospun nanofibers; miRNA deliveries; poly(glycidyl methacrylate)
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Funding
- National Institute of General Medical Science (NIGMS) at the NIH [5P30GM127200-03]
- National Institute of Dental and Craniofacial Research (NIDCR) at the NIH [1R21DE027516, NE LB606]
- University of Nebraska Medical Center
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The healing of large bone defects represents a clinical challenge, often requiring some form of grafting. 3D nanofiber aerogels could be a promising bone graft due to their biomimetic morphology and controlled porous structures and composition. miR-26a has been reported to induce the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and facilitate bone formation. Introducing miR-26a with a suitable polymeric vector targeting BMSCs could improve and enhance the functions of 3D nanofiber aerogels for bone regeneration. Herein, the comb-shaped polycation (HA-SS-PGEA) is first developed, carrying a targeting component, biocleavable groups, and short ethanolamine (EA)-decorated poly(glycidyl methacrylate) (PGMA) (abbreviated as PGEA) arms as miR-26a delivery vector. Thereafter, the cytotoxicity and transfection efficiency of this polycation and cellular response to miR-26a-incorporated nanoparticles (NPs) are assessed in vitro. HA-SS-PGEA exhibits a stronger ability to transport miR-26a and exert its functions than the gold standard polyethyleneimine (PEI) and low-molecular-weight linear PGEA. The efficacy of HA-SS-PGEA/miR-26a NPs loaded 3D hybrid nanofiber aerogels showing a positive effect on the cranial bone defect healing is finally examined. Together, the combination of 3D nanofiber aerogels and functional NPs consisting of a biodegradable and targeting polycation and therapeutic miRNA could be a promising approach for bone regeneration.
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