Journal
ACTA BIOMATERIALIA
Volume 117, Issue -, Pages 108-120Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2020.09.013
Keywords
BMP-2 mimetic peptide; Bone tissue engineering; Injectable hydrogel scaffold; Click-crosslinking; Human dental pulp stem cells; Osteogenic differentiation
Funding
- Creative Materials Discovery Program through the National Research Foundation [2019M3D1A1078938]
- Priority Research Centers Program - National Research Foundation of Korea (NRF) [2019R1A6A1A11051471]
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An injectable, click-crosslinking (Cx) hyaluronic acid (HA) hydrogel scaffold modified with a bone morphogenetic protein-2 (BMP-2) mimetic peptide (BP) was prepared for bone tissue engineering applications. The injectable click-crosslinking HA formulation was prepared from HA-tetrazine (HA-Tet) and HA-cyclooctene (HA-TCO). The Cx-HA hydrogel scaffold was prepared simply by mixing HA-Tet and HA-TCO. The Cx-HA hydrogel scaffold was stable for a longer period than HA both in vitro and in vivo, which was verified via in-vivo fluorescence imaging in real time. BP acted as an osteogenic differentiation factor for human dental pulp stem cells (hDPSCs). After its formation in vivo, the Cx-HA scaffold provided a fine environment for the hDPSCs, and the biocompatibility of the hydrogel scaffold with tissue was good. Like traditional BMP-2, BP induced the osteogenic differentiation of hDPSCs in vitro. The physical properties and injectability of the chemically loaded BP for the Cx-HA hydrogel (Cx-HA-BP) were nearly identical to those of the physically loaded BP hydrogels and the Cx-HA-BP formulation quickly formed a hydrogel scaffold in vivo. The chemically loaded hydrogel scaffold retained the BP for over a month. The Cx-HA-BP hydrogel was better at inducing the osteogenic differentiation of loaded hDPSCs, because it prolonged the availability of BP. In summary, we successfully developed an injectable, click-crosslinking Cx-HA hydrogel scaffold to prolong the availability of BP for efficient bone tissue engineering. (C) 2020 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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