Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 12, Issue 41, Pages 45822-45829Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c13857
Keywords
dual-targeting; single dye; ratiometric polymer nanoprobe; self-calibrating detection; bioimaging
Funding
- NSFC [51603067, 51773056, 21705040]
- Hunan Provincial Natural Science Foundation of China [2020JJ3021]
- China Postdoctoral Science Foundation [2018T110824]
- Scientific Research Fund of Hunan Provincial Education Department [19B204]
- Open Project Program of State Key Laboratory of Chemo/Biosensing and Chemometrics [2018011]
- Open Fund of Hunan Provincial Key Laboratory of Advanced Materials for New Energy Storage and Conversion [2018TP1037-202003]
- Open Fund of the State Key Laboratory of Luminescent Materials and Devices (South China University of Technology) [2019-skllmd-09]
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Exploiting an intelligent fluorescent probe, which can precisely target to the lysosome of hepatoma cells and enable accurate molecular imaging, is a key challenge in hepatoma diagnoses. Herein, a single-dye-based polymer nanoprobe (named SPN) with dual-targeting and self-calibrating ratiometric characteristics is rationally fabricated via a simple self-assembly strategy for accurate hypochlorous acid (HClO) imaging in the lysosome of HepG2 cells. Of note, the covalent incorporation of self-calibrating ratiometric fluorophore (pyrene derivatives) into the core of polymer nanoparticles can not only validly avoid the leakage of fluorophores but also greatly enhance their brightness. Besides, this polymer nanoprobe (SPN) displays high water dispersibility, ultrafast response (<1s), favorable selectivity, outstanding long-term stability (>90 days), and good biocompatibility. Furthermore, thanks to the hepatocyte-targeting moiety (galactose) and the interplay of surface charge and size of nanoparticles, the SPN is able to enter into asialoglycoprotein receptor-positive HepG2 cells and further locate at lysosomes, successfully enabling accurate HClO detection in lysosomes of HepG2 cells. This study demonstrates that the versatile SPN can provide more precise dual-targeting and accurate molecular imaging.
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