Journal
CHEMOSENSORS
Volume 8, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/chemosensors8030063
Keywords
hydrogen peroxide; screen-printed carbon electrode; reduced graphene oxide-based nanocomposite; platinum nanoparticles; real-time monitoring; live cells
Funding
- National Research Foundation - Korean government (MSIP) [NRF-2018R1A2B6007635]
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In this study, we fabricated platinum nanoparticles (PtNP)-decorated, porous reduced graphene oxide (rGO)-carbon nanotube (CNT) nanocomposites on a PtNP-deposited screen-printed carbon electrode (PtNP/rGO-CNT/PtNP/SPCE) for detection of hydrogen peroxide (H2O2), which is released from prostate cancer cells LNCaP. The PtNP/rGO-CNT/PtNP/SPCE was fabricated by a simple electrochemical deposition and co-reduction method. In addition, the amperometric response of the PtNP/rGO-CNT/PtNP/SPCE electrode was evaluated through consecutive additions of H2O2 at an applied potential of 0.2 V (vs. Ag pseudo-reference electrode). As a result, the prepared PtNP/rGO-CNT/PtNP/SPCE showed good electrocatalytic activity toward H2O2 compared to bare SPCE, rGO-CNT/SPCE, PtNP/SPCE, and rGO-CNT/PtNP/SPCE. In addition, the PtNP/rGO-CNT/PtNP/SPCE electrode exhibited a sensitivity of 206 mu A mM(-1).cm(-2) to H2O2 in a linear range of 25 to 1000 mu M (R-2= 0.99). Moreover, the PtNP/rGO-CNT/PtNP/SPCE electrode was less sensitive to common interfering substances, such as ascorbic acid, uric acid, and glucose than H2O2. Finally, real-time monitoring of H2O2 released from LNCaP cells was successfully performed by this electrode. Therefore, we expect that the PtNP/rGO-CNT/PtNP/SPCE can be utilized as a promising electrochemical sensor for practical nonenzymatic detection of H2O2 in live cells or clinical analysis.
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