Journal
PLANTS-BASEL
Volume 9, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/plants9081002
Keywords
Trifolium repens; bacterial endophytes; metals; plant growth promotion; phytoremediation; 16S rRNA gene
Categories
Funding
- BOF Special Research Fund from Hasselt University
- UHasselt Methusalem project [08M03VGRJ]
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Heavy metals in soil, as selective agents, can change the structure of plant-associated bacterial communities and their metabolic properties, leading to the selection of the most-adapted strains, which might be useful in phytoremediation.Trifolium repens, a heavy metal excluder, naturally occurs on metal mine waste heaps in southern Poland characterized by high total metal concentrations. The purpose of the present study was to assess the effects of toxic metals on the diversity and metabolic properties of the microbial communities in rhizospheric soil and vegetative tissues ofT. repensgrowing on three 70-100-years old Zn-Pb mine waste heaps in comparison toTrifolium-associated bacteria from a non-polluted reference site. In total, 113 cultivable strains were isolated and used for 16S rRNA gene Sanger sequencing in order to determine their genetic affiliation and for in vitro testing of their plant growth promotion traits. Taxa richness and phenotypic diversity in communities of metalliferous origin were significantly lower (p< 0.0001) compared to those from the reference site. Two strains,Bacillus megateriumBolR EW3_A03 andStenotrophomonas maltophiliaBolN EW3_B03, isolated from a Zn-Pb mine waste heap which tested positive for all examined plant growth promoting traits and which showed co-tolerance to Zn, Cu, Cd, and Pb can be considered as potential facilitators of phytostabilization.
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