Journal
ANTIBIOTICS-BASEL
Volume 9, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/antibiotics9060302
Keywords
LC-ESI-MS/MS; proteomics; mass spectrometry; characterization of antibiotic resistance peptides; lantibiotic production; virulence factors; Streptococcus spp
Categories
Funding
- Xunta de Galicia
- European Union Social Fund (ESF)
- Spanish Ministry of Economy and Competitiveness [AGL 2.013-48.244-R]
- European Regional Development Fund (ERDF)
Ask authors/readers for more resources
Streptococcusspp. are major mastitis pathogens present in dairy products, which produce a variety of virulence factors that are involved in streptococcal pathogenicity. These include neuraminidase, pyrogenic exotoxin, and M protein, and in addition they might produce bacteriocins and antibiotic-resistance proteins. Unjustifiable misuse of antimicrobials has led to an increase in antibiotic-resistant bacteria present in foodstuffs. Identification of the mastitis-causing bacterial strain, as well as determining its antibiotic resistance and sensitivity is crucial for effective therapy. The present work focused on the LC-ESI-MS/MS (liquid chromatography-electrospray ionization tandem mass spectrometry) analysis of tryptic digestion peptides from mastitis-causingStreptococcusspp. isolated from milk. A total of 2706 non-redundant peptides belonging to 2510 proteins was identified and analyzed. Among them, 168 peptides were determined, representing proteins that act as virulence factors, toxins, anti-toxins, provide resistance to antibiotics that are associated with the production of lantibiotic-related compounds, or play a role in the resistance to toxic substances. Protein comparisons with the NCBI database allowed the identification of 134 peptides as specific toStreptococcusspp., while two peptides (EATGNQNISPNLTISNAQLNLEDKNK and DLWC*NM*IIAAK) were found to be species-specific toStreptococcus dysgalactiae. This proteomic repository might be useful for further studies and research work, as well as for the development of new therapeutics for the mastitis-causingStreptococcusstrains.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available