Journal
ANTIBIOTICS-BASEL
Volume 9, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/antibiotics9040192
Keywords
Escherichia coli; ESBL; hybrid pathotype; ExPEC; EPEC; MDR; ST10; O153; EnteroBase
Categories
Funding
- Ministerio de Economia y Competitividad (MINECO, Spain) [AGL2013-47852-R]
- Fondo Europeo de Desarrollo Regional (FEDER)
- Agencia Estatal de Investigacion (AEI, Spain) [AGL2016-79343-R]
- FEDER
- Plan Estatal de I+D+I 2013-2016 [PI16/01477]
- Instituto de Salud Carlos III (ISCIII)
- Subdireccion General de Evaluacion y Fomento de la Investigacion
- Conselleria de Cultura, Educacion e Ordenacion Universitaria of Xunta de Galicia [ED431C2017/57]
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Different surveillance studies (2005-2015) in northwest Spain revealed the presence of eae-positive isolates of Escherichia coli O153:H10 in meat for human consumption, poultry farm, wildlife and human diarrheagenic samples. The aim of this study was to explore the genetic and genomic relatedness between human and animal/meat isolates, as well as the mechanism of its persistence. We also wanted to know whether it was a geographically restricted lineage, or whether it was also reported elsewhere. Conventional typing showed that 32 isolates were O153:H10-A-ST10 fimH54, fimAv(MT78), traT and eae-beta1. Amongst these, 21 were CTX-M-32 or SHV-12 producers. The PFGE XbaI-macrorestriction comparison showed high similarity (>85%). The plasmidome analysis revealed a stable combination of IncF (F2:A-:B-), IncI1 (STunknown) and IncX1 plasmid types, together with non-conjugative Col-like plasmids. The core genome investigation based on the cgMLST scheme from EnteroBase proved close relatedness between isolates of human and animal origin. Our results demonstrate that a hybrid MDR aEPEC/ExPEC of the clonal group O153:H10-A-ST10 (CH11-54) is circulating in our region within different hosts, including wildlife. It seems implicated in human diarrhea via meat transmission, and in the spreading of ESBL genes (mainly of CTX-M-32 type). We found genomic evidence of a related hybrid aEPEC/ExPEC in at least one other country.
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