Journal
MICROORGANISMS
Volume 8, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms8081180
Keywords
abdominal fats; butyric acid; high-fat diet; Leuconostoc mesenteroides; PPAR-gamma
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Funding
- Landseed Hospital-NCU joint grants [106/107/108]
- Ministry of Science and Technology (MOST) [108-2622-B-008-001-CC1, 108-2314-B-008-003-MY3, 107-2923-B-008-001-MY3]
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The activation of peroxisome proliferator-activated rece ptor gamma (PPAR-gamma) is known to induce the differentiation of adipocytes. This study aimed to investigate the probiotic effect ofLeuconostoc mesenteroides(L. mesenteroides) on high-fat diet (HFD)-induced PPAR-gamma activation and abdominal fat depots. Incubation of differentiated 3T3-L1 adipocytes with media ofL. mesenteroidesEH-1, a butyric acid-producing strain, significantly reduced the amounts of lipid droplets. The oral administration ofL. mesenteroidesEH-1 produced large amounts (>1 mM) of butyric acid in cecum and attenuated the HFD-induced upregulation of PPAR-gamma and accumulation of abdominal fats in mice. The combination of 2% glucose withL. mesenteroidesEH-1 increased the production of butyric acid and potentiated the probiotic activity ofL. mesenteroidesEH-1 against the formation of lipid droplets in 3T3-L1 adipocytes as well as abdominal fats in HFD-fed mice. The inhibition of free fatty acid receptor 2 (Ffar2) by its antagonist, GLPG-0974, markedly diminished the probiotic effects ofL. mesenteroidesEH-1 plus glucose on the suppression of HFD-induced PPAR-gamma and abdominal fats. Besides demonstrating the probiotic value ofL. mesenteroidesEH-1, our results highlight the possible therapy targeting the butyric acid-activated Ffar2 pathway to reduce abdominal fats.
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