Journal
ANTIOXIDANTS
Volume 9, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/antiox9060485
Keywords
ethosome; ubiquinone; H2O2; penetration enhancers; dermal administration; reconstituted human epidermis; small angle X-ray scattering
Funding
- University of Ferrara (FIR 2019)
- University of Ferrara (FAR 2019)
- European Union`s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant [765602]
- Marie Curie Actions (MSCA) [765602] Funding Source: Marie Curie Actions (MSCA)
Ask authors/readers for more resources
Ethosome represents a smart transdermal vehicle suitable for solubilization and cutaneous application of drugs. Coenzyme Q10 is an endogenous antioxidant whose supplementation can counteract many cutaneous disorders and pathologies. In this respect, the present study describes the production, characterization, and cutaneous protection of phosphatidylcholine based ethosomes as percutaneous delivery systems for coenzyme Q10. CoQ10 entrapment capacity in ethosomes was almost 100%, vesicles showed the typical 'fingerprint' structure, while mean diameters were around 270 nm, undergoing an 8% increase after 3 months from production. An ex-vivo study, conducted by transmission electron microscopy, could detect the uptake of ethosomes in human skin fibroblasts and the passage of the vesicles through 3D reconstituted human epidermis. Immunofluorescence analyses were carried on both on fibroblasts and 3D reconstituted human epidermis treated with ethosomes in the presence of H(2)O(2)as oxidative stress challenger, evaluating 4-hydroxynonenal protein adducts which is as a reliable biomarker for oxidative damage. Notably, the pretreatment with CoQ10 loaded in ethosomes exerted a consistent protective effect against oxidative stress, in both models, fibroblasts and in reconstituted human epidermis respectively.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available