Journal
SCIENCE IMMUNOLOGY
Volume 5, Issue 48, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciimmunol.aaz8035
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Funding
- Australian Research Council [CE140100011]
- National Health and Medical Research Council (NHMRC) [1113293]
- New Zealand Ministry of Business Innovation and Employment [RTVU1603]
- Avalia Immunotherapies
- NHMRC [1117766, 1154457]
- National Health and Medical Research Council of Australia [1154457, 1117766] Funding Source: NHMRC
- New Zealand Ministry of Business, Innovation & Employment (MBIE) [RTVU1603] Funding Source: New Zealand Ministry of Business, Innovation & Employment (MBIE)
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Liver resident-memory CD8(+) T cells (T-RM cells) can kill liver-stage Plasmodium-infected cells and prevent malaria, but simple vaccines for generating this important immune population are lacking. Here, we report the development of a fully synthetic self-adjuvanting glycolipid-peptide conjugate vaccine designed to efficiently induce liver T-RM cells. Upon cleavage in vivo, the glycolipid-peptide conjugate vaccine releases an MHC I-restricted peptide epitope (to stimulate Plasmodium-specific CD8(+) T cells) and an adjuvant component, the NKT cell agonist alpha-galactosylceramide (alpha-GalCer). A single dose of this vaccine in mice induced substantial numbers of intrahepatic malaria-specific CD8(+) T cells expressing canonical markers of liver T-RM cells (CD69, CXCR6, and CD101), and these cells could be further increased in number upon vaccine boosting. We show that modifications to the peptide, such as addition of proteasomal-cleavage sequences or epitope-flanking sequences, or the use of alternative conjugation methods to link the peptide to the glycolipid improved liver T-RM cell generation and led to the development of a vaccine able to induce sterile protection in C57BL/6 mice against Plasmodium berghei sporozoite challenge after a single dose. Furthermore, this vaccine induced endogenous liver T-RM cells that were long-lived (half-life of similar to 425 days) and were able to maintain >90% sterile protection to day 200. Our findings describe an ideal synthetic vaccine platform for generating large numbers of liver T-RM cells for effective control of liver-stage malaria and, potentially, a variety of other hepatotropic infections.
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