The complete mitochondrial genome analysis of a stonefly,Acroneuria carolinensis(Plecoptera: Perlidae)

We sequenced and annotated the species ofAcroneuria carolinensiswhich is the second species representative of the genusAcroneuriato provide mitochondrial genome data for order Plecoptera. The complete mitochondrial genome ofA. carolinensiswas 15,718 bp in length and possessed 22 transfer RNA (tRNA) genes, 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes and one control region. The A + T account of the whole genome, PCGs, tRNAs, rRNAs, and the control region was 64.6%, 62.7%, 67.8%, 68.6%, and 73.9%, respectively. Ten PCGs used the normal start codon exceptND2,ND5, andND1used the exceptional GTG and TTG as start codon. All PCGs stopped with the terminal codon TAN, but onlyCOIIgene used the single T--. The phylogenetic tree was based on the PCG12R matrix (including PCGs first and second codons and two rRNAs) by Bayesian inference (BI) method and we found that the genusAcroneuriaandSinacroneuriawere clustered in a clade.