Journal
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
Volume 18, Issue -, Pages 2209-2216Publisher
ELSEVIER
DOI: 10.1016/j.csbj.2020.06.038
Keywords
Fluorescence polarization microscopy; Dipole orientation; Super-resolution; Biomolecule interactions; Molecular organization
Funding
- National Natural Science Foundation of China [31671383, 81890991, 61773230, 61721003]
- State Key Research Development Program of China [2017YFA0505503]
- Beijing Advanced Innovation Center for Structural Biology, Tsinghua University
- Foshan-Tsinghua Innovation Special Fund [FTISF 2019THFS0141]
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Fluorescence polarization microscopy (FPM) analyzes both intensity and orientation of fluorescence dipole, and reflects the structural specificity of target molecules. It has become an important tool for studying protein organization, orientational order, and structural changes in cells. However, suffering from optical diffraction limit, conventional FPM has low orientation resolution and observation accuracy, as the polarization information is averaged by multiple fluorescent molecules within a diffraction-limited volume. Recently, novel super-resolution FPMs have been developed to break the diffraction barrier. In this review, we will introduce the recent progress to achieve sub-diffraction determination of dipole orientation. Biological applications, based on polarization analysis of fluorescence dipole, are also summarized, with focus on chromophore-target molecule interaction and molecular organization. (C) 2020 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology.
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