Journal
ACS SENSORS
Volume 5, Issue 9, Pages 2806-2813Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.0c00880
Keywords
pantetheinase detection; NIR emission ratiometric; mice imaging; fast and efficient response; associated with diabetes
Funding
- Natural Science Foundation of China [21771092, 81903561]
- Fundamental Research Funds for the Central Universities [lzujbky-2020-kb13]
- Special Fund Project of Guiding Scientific, Technological Innovation Development of Gansu Province [2019ZX-04]
- 111 Project [B20027]
- State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China
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Pantetheinase (Vann -1) is an ectoenzyme, which involves the metabolic pathway of coenzyme A (CoA), and can decompose pantetheine into pantothenic acid (CoA precursor) and aminothiol cysteamine. Previous studies have revealed that Vanin-1 with essential biological functions is closely related to many diseases. However, the lack of simple and effective detection methods has severely hindered the further study of Vanin-l's physiological ftinctions. In this work, we have developed a nearinfrared (NIR) emission ratio fluorescent probe TMN-PA (I-645 nm/I-568 (nm)) that enables us to detect Vanin-1 rapidly (in 15 min) with a minimum detection limit of 0.37 ng/mL. What is more, this probe shows excellent potential in in situ real-time monitoring of the endogenous Vanin-1, contributing to further research on Vanm-1 and understanding its mechanisms in physiological pathology. To our knowledge, this probe is the first NIR emission ratio (I-645 nm/I-568 (nm)) fluorescent probe ever reported to monitor the activity of Vanin-1 in vivo.
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