Journal
CELL SYSTEMS
Volume 11, Issue 2, Pages 145-+Publisher
CELL PRESS
DOI: 10.1016/j.cels.2020.06.011
Keywords
-
Categories
Funding
- National Institutes of Health [DP2-GM-119138, 1R35GM134886]
- Alfred P. Sloan Foundation [FG-2016-6229]
- Pew Charitable Trusts [00029624]
- UC-Berkeley
- Bowes Foundation
- NSF predoctoral fellowship [DGE 1752814]
- NIH [T32 GM 0007232, T32 HG 00047, R35-GM-128802]
- Columbia startup funding
- National Human Genome Research Institute of the National Institutes of Health [U41HG007234, HG004037]
Ask authors/readers for more resources
Genomic analyses in budding yeast have helped define the foundational principles of eukaryotic gene expression. However, in the absence of empirical methods for defining coding regions, these analyses have historically excluded specific classes of possible coding regions, such as those initiating at non-AUG start codons. Here, we applied an experimental approach to globally annotate translation initiation sites in yeast and identified 149 genes with alternative N-terminally extended protein isoforms initiating from near-cognate codons upstream of annotated AUG start codons, These isoforms are produced in concert with canonical isoforms and translated with high specificity, resulting from initiation at only a small subset of possible start codons. The non-AUG initiation driving their production is enriched during meiosis and induced by low elF5A, which is seen in this context. These findings reveal widespread production of non-canonical protein isoforms and unexpected complexity to the rules by which even a simple eukaryotic genome is decoded.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available