Journal
APPLIED SCIENCES-BASEL
Volume 10, Issue 13, Pages -Publisher
MDPI
DOI: 10.3390/app10134593
Keywords
biodegradation; dehalogenase-producing bacteria; haloalkanoic acids; trichloroacetic acid; 16S rRNA; Lysinibacillus
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Funding
- European Union (European Structural and Investment Funds Operational Program Research, Development, and Education) [CZ.02.1.01/0.0/0.0/16_025/0007293]
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Halogenated compounds are an important class of environmental pollutants that are widely used in industrial chemicals such as solvents, herbicides, and pesticides. Many studies have been carried out to explore the biodegradation of these chemicals. Trichloroacetic acid (TCA) is one of the main halogenated compounds that are carcinogenic to humans and animals. The bacterium was isolated from the northern coastline of Johor Strait. In this study, the ability of strain MH2 to biodegrade TCA was evaluated by a growth experiment and dehalogenase enzyme assay. The growth profile of the isolated strain was examined. The doubling time forL. boronitoleransMH2 was found to be 32 h. The release of chloride ion in the degradation process was measured at 0.33 x 10(-3)+/- 0.03 mol center dot L(-1)after 96 h when the growth curve had reached its maximum within the late bacterial exponential phase. The results showed that the strain had a promising ability to degrade TCA by producing dehalogenase enzyme when cell-free extracts were prepared from growth on TCA as the sole carbon source with enzyme-specific activity, 1.1 +/- 0.05 mu molCl(-)min(-1)center dot mg(-1)protein. Furthermore, the morphological, and biochemical aspects of the isolated bacterium were studied to identify and characterize the strain. The morphological observation of the isolated bacterium was seen to be a rod-shaped, Gram-positive, motile, heterotrophic, and spore-forming bacterium. The amplification of the 16S rRNA and gene analysis results indicated that the isolated bacterium had 98% similarity toLysinibacillus boronitolerans. The morphological and biochemical tests supported the 16S rRNA gene amplification. To the best of the authors' knowledge, this is the first reported case of this genus of bacteria to degrade this type of halogenated compound.
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