Journal
STEM CELL REPORTS
Volume 15, Issue 3, Pages 735-748Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2020.07.009
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Funding
- Arrow Bone Marrow Transplant Foundation
- St. Vincent's Clinic Foundation
- St. Vincent's Hospital Sydney Hematology Department Research Funds
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Induced pluripotent stem cells (iPSCs) are an invaluable resource for the study of human disease. However, there are no standardized methods for differentiation into hematopoietic cells, and there is a lack of robust, direct comparisons of different methodologies. In the current study we improved a feeder-free, serum-free method for generation of hematopoietic cells from iPSCs, and directly compared this with three other commonly used strategies with respect to efficiency, repeatability, hands-on time, and cost. We also investigated their capability and sensitivity to model genetic hematopoietic disorders in cells derived from Down syndrome and beta-thalassemia patients. Of these methods, a multistep monolayer-based method incorporating aryl hydrocarbon receptor hyperactivation (2D-multistep) was the most efficient, generating significantly higher numbers of CD34(+) progenitor cells and functional hematopoietic progenitors, while being the most timeand cost-effective and most accurately recapitulating phenotypes of Down syndrome and beta-thalassemia.
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