Journal
STEM CELL REPORTS
Volume 15, Issue 1, Pages 13-21Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2020.05.011
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Funding
- Braukmann-Wittenberg-Herz-Stiftung
- Deutsche Forschungsgemeinschaft
- Deutsche Forschungsgemeinschaft, Germany [DFG: ZW64/4-1, REBIRTH DFG EXC62/2, EXC62/3, KFO311 ZW64/7-1]
- Federal Ministry for Education and Science, Germany [13N14086, 01EK1601A, 01EK1602A, 13XP5092B]
- European Union H2020 program (TECHNOBEAT grant) [66724]
- Federal Ministry of Education and Research, Germany [01KL1903]
- European Research Council, (consolidator grant Longheart, LS4, ERC-2014-CoG)
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Pluripotency is tightly regulated and is crucial for stem cells and their implementation for regenerative medicine. Non-coding RNAs, espe-cially long non -coding RNAs (lncRNAs) emerged as orchestrators of versatile (patho)-physiological processes on the transcriptional and post-transcriptional level. Cyrano , a well-conserved lncRNA, is highly expressed in stem cells suggesting an important role in pluripo-tency, which we aimed to investigate in loss-off-function (LOF) experiments. Cyrano was described previously to be essential for the main- tenance of mouse embryonic stem cell (ESC) pluripotency. In contrast, using different genetic models, we here found Cyrano to be dispensable in murine and human iPSCs and in human ESCs. RNA sequencing revealed only a moderate influence of Cyrano on the global transcriptome. In line, Cyrano-depleted iPSCs retained the potential to differentiate into the three germ layers. In conclusion, different methods were applied for LOF studies to rule out potential off-target effects. These approaches revealed that Cyrano does not impact pluripotency.
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