Journal
CATALYSTS
Volume 10, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/catal10070730
Keywords
penicillin acylase; aculeacin acylase; N-acyl-homoserine lactone acylases; quorum quenching; biofouling
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Funding
- Ministry of Education and Science [BIO2008-03928]
- Ministry of Science and Innovation of Spain [DEX-580000-2008-31]
- Comunidad Autonoma de Madrid [S2009/PPQ-1752]
- Ministry of Economy, Industry and Competitiveness of Spain [CTQ2014-60250-R, CTM2016-76491-P]
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Many Gram-negative bacteria produceN-acyl-homoserine lactones (AHLs), quorum sensing (QS) molecules that can be enzymatically inactivated by quorum quenching (QQ) processes; this approach is considered an emerging antimicrobial alternative. In this study, kinetic parameters of several AHLs hydrolyzed by penicillin acylase fromStreptomyces lavendulae(SlPA) and aculeacin A acylase fromActinoplanes utahensis(AuAAC) have been determined. Both enzymes catalyze efficiently the amide bond hydrolysis in AHLs with different acyl chain moieties (with or without 3-oxo modification) and exhibit a clear preference for AHLs with long acyl chains (C12-HSL > C14-HSL > C10-HSL > C8-HSL forSlPA, whereas C14-HSL > C12-HSL > C10-HSL > C8-HSL forAuAAC). Involvement ofSlPA andAuAAC in QQ processes was demonstrated byChromobacterium violaceumCV026-based bioassays and inhibition of biofilm formation byPseudomonas aeruginosa, a process controlled by QS molecules, suggesting the application of these multifunctional enzymes as quorum quenching agents, this being the first time that quorum quenching activity was shown by an aculeacin A acylase. In addition, a phylogenetic study suggests thatSlPA andAuAAC could be part of a new family of actinomycete acylases, with a preference for substrates with long aliphatic acyl chains, and likely involved in QQ processes.
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