4.3 Article

Acinar transformed ductal cells exhibit differential mucin expression in a tamoxifen-induced pancreatic ductal adenocarcinoma mouse model

Journal

BIOLOGY OPEN
Volume 9, Issue 9, Pages -

Publisher

COMPANY BIOLOGISTS LTD
DOI: 10.1242/bio.052878

Keywords

Mucin; Pancreatic ductal adenocarcinoma (PDAC); Inducible KC (iKC) mouse model

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Funding

  1. National Institutes of Health, National Cancer Institute [P01 CA217798 R21 CA238953, CA183459, R01 CA 195586, R01 CA206444]

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Pancreatic cancer (PC) is acquired postnatally; to mimic this scenario, we developed an inducible Kras(G12D); Ptf1a-CreER (TM) (iKC) mouse model, in which Kras is activated postnatally at week 16 upon tamoxifen (TAM) administration. Upon TAM treatment, iKC mice develop pancreatic intraepithelial neoplasia (PanIN) lesions and PC with metastasis at the fourth and fortieth weeks, respectively, and exhibited acinar-to-ductal metaplasia (ADM) and transdifferentiation. Kras activation upregulated the transcription factors Ncoa3, p-cJun and FoxM1, which in turn upregulated expression of transmembrane mucins (Muc1, Muc4 and Muc16) and secretory mucin (Muc5Ac). Interestingly, knockdown of Kras(G)(12)(D) in multiple PC cell lines resulted in downregulation of MUC1, MUC4, MUC5AC and MUC16. In addition, iKC mice exhibited ADM and transdifferentiation. Our results show that the iKC mouse more closely mimics human PC development and can be used to investigate pancreatic ductal adenocarcinoma (PDAC) biomarkers, early onset of PDAC, and ADM. The iKC model can also be used for preclinical strategies such as targeting mucin axis alone or in combination with neo-adjuvant, immunotherapeutic approaches and to monitor chemotherapy response.

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