Reverse expression pattern of sirtuin-1 and histone deacetylase-9 in coronary artery disease

Background SIRT1andHDAC9 genes are related to inflammation and may contribute to the pathogenesis of coronary artery disease (CAD). We aimed to evaluate the expression level, methylation profile and polymorphisms of these genes in CAD patients. Methods In this study, 50 CAD patients and 50 healthy individuals were recruited. The expression level change was evaluated using the TaqMan Real-Time PCR method. The methylation of genes promoter and genotyping of polymorphisms were evaluated by the HRM. Results The expression level ofSIRT1was reduced while theHDAC9expression level showed a significant elevation (p < .001). TheSIRT1gene promoter was hypomethylated and theHDAC9gene promoter was hypermethylated in CAD patients. Also, CG + GG genotype inSIRT1and both genotypes in theHDAC9gene were associated with expression change. Conclusions SIRT1andHDAC9genes, expression changes can be suggested as a potential biomarker for CAD detection.

Automated summary

This study evaluated the expression level, methylation profile, and polymorphisms of SIRT1 and HDAC9 genes in CAD patients. The results suggest that the expression changes of these genes may serve as potential biomarkers for CAD detection.