Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 144, Issue -, Pages 250-256Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2016.04.018
Keywords
Isothermal Titration Calorimetry; Receptor-ligand binding; Thermodynamics; Cell adhesion; blood brain barrier
Funding
- National Operative Program Research and Competitiveness [Pon a3_00173-CeSmeMo]
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Targeted therapies represent a challenge in modern medicine. In this contest, we propose a rapid and reliable methodology based on Isothermal Titration Calorimetry (ITC) coupled with confluent cell layers cultured around biocompatible templating microparticles to quantify the number of overexpressing receptors on cell membrane and study the energetics of receptor-ligand binding in near-physiological conditions. In the in vitro model here proposed we used the bEnd3 cell line as brain endothelial cells to mimic the blood brain barrier (BBB) cultured on dextran microbeads ranging from 67 mu m to 80 mu m in size (Cytodex) and the primary human umbilical vein cells (HUVEC) for comparison. The revealed affinity between transferrin (Tf) and transferrin receptor (TfR) in both systems is very high, Kd values are in the order of nM. Conversely, the value of TfRs/cell reveals a 100-fold increase in the number of TfRs per bEnd3 cells compared to HUVEC cells. The presented methodology can represent a novel and helpful strategy to identify targets, to address drug design and selectively deliver therapeutics that can cross biological barriers such as the blood brain barrier. (C) 2016 Elsevier B.V. All rights reserved.
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