Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 137, Issue -, Pages 2-16Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2015.07.048
Keywords
Escherichia coli; Active colloids; Motility; Differential dynamic microscopy; Metabolism; Bioenergetics; Proton motive force
Funding
- EPSRC Senior Research Fellowship [EP/D071070]
- EPSRC [EP/J007404/1]
- ERC Advanced Grant (ADG-PHYAPS)
- Marie Curie Fellowship 'ActiveDynamics' FP7-PEOPLE [PIIF-GA-2010-276190]
- EPSRC
- Marie Curie fellowship 'Living Patchy Colloids' (LivPaC) under FP7-PEOPLE-2013-IEF program [623364]
- Edinburgh Chancellor's Fellowship
- BBSRC/EPSRC/MRC Synthetic Biology Research Centre [BB/M018040/1]
- BBSRC [BB/M018040/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M018040/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/J007404/1] Funding Source: researchfish
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The flagellated bacterium Escherichia coli is increasingly used experimentally as a self-propelled swimmer. To obtain meaningful, quantitative results that are comparable between different laboratories, reproducible protocols are needed to control, 'tune' and monitor the swimming behaviour of these motile cells. We critically review the knowledge needed to do so, explain methods for characterising the colloidal and motile properties of E. coli cells, and propose a protocol for keeping them swimming at constant speed at finite bulk concentrations. In the process of establishing this protocol, we use motility as a high-throughput probe of aspects of cellular physiology via the coupling between swimming speed and the proton motive force. (C) 2015 Elsevier B.V. All rights reserved.
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