Enzyme immobilization on a nanoadsorbent for improved stability against heavy metal poisoning

Magnetic nanoparticles modified with siloxane layers bearing amino and thiol functions have been used for immobilization of urease either by adsorption or via surface grafting. The activity of the immobilized enzyme in the hydrolysis of urea extended to the levels typical of the native enzyme, while its long-term stability in combination with magnetic retraction opened for its repeated use in both analysis and detoxification of bio-fluids. The immobilized urease revealed strongly enhanced stability and 65% activity in the presence of 0.1 mmol/l of Hg2+ or 0.3 mmol/l of Cu2+ while the native urease did not retain any activity at all. The enzyme grafting was shown to be a potentially perspective tool in alleviation of heavy metal poisoning and to be providing an opportunity for use of the developed adsorbents as both biosensors and bio-reactants for removal of urea from biofluids. (C) 2016 Elsevier B.V. All rights reserved.