Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 141, Issue -, Pages 397-407Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2016.01.033
Keywords
Casein; Layer-by-layer self-assembly; Multilayer films; Stem cell; Osteogenic differentiation
Funding
- National Natural Science Foundation of China [51303216, 51103182, 51203194]
- Guangdong Provincial Education Department [2013KJCX005]
- Science and Technology Planning Project of Guangdong Province [2011A060901013, 2015A030313144]
- Science and Technology Program of Guangzhou [2060204, 201300000057, 20153900042050200, 2014Y2-00227]
- ITI grant research
- Guangdong Innovative Research Team Program [2009010057]
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Creating a bioactive surface is important in tissue engineering. Inspired by the natural calcium binding property of casein (CA), multilayer films ((CA/CS)(n)) with chitosan (CS) as polycation were fabricated to enhance biomineralization, cell adhesion and differentiation. LBL self-assembly technique was used and the assembly process was intensively studied based on changes of UV absorbance, zeta potential and water contact angle. The increasing content of chitosan and casein with bilayers was further confirmed with XPS and TOF-SIMS analysis. To improve the biocompatibility, gelatin was surface grafted. In vitro mineralization test demonstrated that multilayer films had more hydroxyapatite crystal deposition. Human mesenchymal stem cells (HMSCs) were seeded onto these films. According to fluorescein diacetate (FDA) and cell cytoskeleton staining, MTF assay, expression of osteogenic marker genes, ALP activity, and calcium deposition quantification, it was found that these multilayer films significantly promoted HMSCs attachment, proliferation and osteogenic differentiation than TCPS control. (C) 2016 Published by Elsevier B.V.
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