Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 137, Issue -, Pages 104-108Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2015.04.062
Keywords
Peptides; Peptide conjugates; Self-assembly; Fluorescence
Funding
- BBSRC [BB/I008187/1]
- EPSRC [EP/L020599/1]
- BBSRC [BB/I008187/2] Funding Source: UKRI
- STFC [ST/L502480/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/I008187/1, BB/I008187/2] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/L020599/1] Funding Source: researchfish
- Science and Technology Facilities Council [1498302, ST/L502480/1] Funding Source: researchfish
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Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in pen-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.orgflicenses/by/4.0/).
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