Journal
FRONTIERS IN MICROBIOLOGY
Volume 11, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2020.01604
Keywords
high-fat diet; heavy metal; gut microbiota; arsenic; cadmium; lead
Categories
Funding
- Natural Science Foundation of Guangdong Province (NSFG) [2018A030310168, 2020A151501910]
- National Natural Science Foundation of China (NSFC) [81703333]
- Industry-University-Research Cooperation Foundation of Guangdong Science and Technology Department (IUCF-GSTD) [2017A090905038]
- Guangzhou Key Laboratory Fund [201905010004]
Ask authors/readers for more resources
Previous studies proved that heavy metals could increase the risk of disease by acting on the gut microbiota. Meanwhile, gut microbiota played important roles in detoxifying heavy metals. However, the response of gut microbiota to heavy metals and which microbes dominated this detoxification processes are still unclear. This study investigated the difference of high-fat-diet (HFD) and normal-diet (ND) gut microbiota and their response to and detoxification effects on arsenic (As), cadmium (Cd), and lead (Pb) exposure. Results showed that gut microbiota of ND and HFD was significantly different and responded to As, Pb, and Cd exposure differently, too. When exposed to 100 ppm As, Cd, or Pb, HFD-fed mice accumulated more heavy metals in the liver and kidney along with more severe functional damage than ND-fed mice, indicated by a more dramatic increase of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and urinary total protein (TPU), urinary uric acid (UUA), and urinary creatinine (Ucrea) content. Among ND gut microbiota, relative abundance ofBacteroides,Lactobacillus,Butyricimonas, andDoreawas significantly increased by arsenic (As) exposure; relative abundance ofFaecoccusandLactobacilluswas significantly increased by Cd exposure; relative abundance ofDesulfovibrio,Plasmodium, andRoseburiawere significantly increased by Pb exposure. However, among HFD gut microbiota, those microbes were not significantly changed. Bivariate association analysis found weak positive correlations between content of fecal excreted heavy metals and richness of total fecal microbiota as well as abundance of some of the heavy metal-enriched microbes. Our study concluded that HFD increased disease risk of heavy metal exposure probably via its gut microbiota which excreted less heavy metal through feces.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available