Journal
FRONTIERS IN MICROBIOLOGY
Volume 11, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2020.01645
Keywords
prokaryotic communities; marine particles; size-fractionation; in situpumps; oceanographic bottles; polynyas
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Funding
- Edith Cowan University [G1003456]
- School of Science at Edith Cowan University [G1003362]
- Spanish Ministry of Science, Innovation and Universities [CTM2015-70340-R, RTI2018-101025-B-I00]
- Generalitat de Catalunya Consolidated Research Group [2017SGR/1568]
- Generalitat de Catalunya [MERS 2017 SGR -1588]
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Microbes associated with sinking marine particles play key roles in carbon sequestration in the ocean. The sampling of particle-attached microorganisms is often done with sediment traps or by filtration of water collected with oceanographic bottles, both involving a certain time lapse between collection and processing of samples that may result in changes in particle-attached microbial communities. Conversely,in situwater filtration through submersible pumps allows a faster storage of sampled particles, but it has rarely been used to study the associated microbial communities and has never been compared to other particle-sampling methods in terms of the recovery of particle microbial diversity. Here we compared the prokaryotic communities attached to small (1-53 mu m) and large (>53 mu m) particles collected from the mesopelagic zone (100-300 m) of two Antarctic polynyas usingin situpumps (ISP) and oceanographic bottles (BTL). Each sampling method retrieved largely different particle-attached communities, suggesting that they capture different kinds of particles. These device-driven differences were greater for large particles than for small particles. Overall, the ISP recovered 1.5- to 3-fold more particle-attached bacterial taxa than the BTL, and different taxonomic groups were preferentially recovered by each method. In particular, typical particle-attached groups such as Planctomycetes and Deltaproteobacteria recovered with ISP were nearly absent from BTL samples. Our results suggest that the method used to sample marine particles has a strong influence in our view of their associated microbial communities.
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