Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 147, Issue -, Pages 36-44Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2016.07.005
Keywords
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Funding
- Fundacao para a Ciencia e Tecnologia [SFRH/BD/85690/2012, SFRH/BPD/89982/2012]
- FCT/MCTES (Portugal)
- Research Unit GREEN-it Bioresources for Sustainability [UID/Multi/04551/2013]
- CICECO-Aveiro Institute of Materials [FCTUID/CTM/50011/2013]
- [FCOMP-01-0124-FEDER-041484]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/85690/2012] Funding Source: FCT
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Amyloid fibrils have recently gained a lot of attention due to their morphology, functionality and mechanical strength, allowing for their application in nanofiber-based materials, biosensors, bioactive membranes and tissue engineering scaffolds. The in vitro production of amyloid fibrils is still a slow process, thus hampering the massive production of nanofibers and its consequent use. This work presents a new and faster (2-3 h) fibrillation method for hen egg white lysozyme (HEWL) using a deep eutectic solvent based on cholinium chloride and acetic acid. Nanofibers with dimensions of 0.5-1 mu m in length and 0.02-0.1 mu m in thickness were obtained. Experimental variables such as temperature and pH were also studied, unveiling their influence in fibrillation time and nanofibers morphology. These results open a new scope for protein fibrillation into nanofibers with applications ranging from medicine to soft matter and nanotechnology. (C) 2016 Elsevier B.V. All rights reserved.
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