Journal
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
Volume 510, Issue -, Pages 183-189Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfa.2016.07.078
Keywords
Gold nanoparticles; Biocatalytic enlargement; Glucose oxidase; UV-vis spectroscopy; Dynamic light scattering; Atomic force microscopy
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The aim of this study was the investigation of the biocatalytic enlargement of colloidal gold nanoparticles (AuNPs) using enzyme glucose oxidase (GOx), glucose and tetrachloroauric acid by UV-vis spectroscopy, dynamic light scattering (DLS) and atomic force microscopy (AFM). The appropriate ratio of 13 nm AuNPs seeds and growing solutions equal to 1:2 was determined from absorbance spectra of AuNPs. At this ratio the most AuNP seeds were biocatalytically enlarged and it resulted in the higher difference of plasmon absorbance after 60 and 90 min of AuNPs incubation in growing solution. In order to determine the size of AuNPs after biocatalytic enlargement, DLS measurements in the solution and AFM measurements after the immobilization of AuNPs seeds on sensor disc coated with a planar Au layer, which was functionalized with 1,6-hexanedithiol self-assembled monolayer, were performed. The DLS and AFM measurements showed that hydrodynamic diameter of biocatalytically enlarged AuNPs in the solution and the height of nanoparticles immobilized on the surface depends on the glucose concentration in the solution and on the duration of synthesis. Therefore, the biocatalytic enlargement of AuNPs could be applied in the development of glucose biosensors using UV-vis spectroscopy, DLS and AFM methods. (C) 2016 Elsevier B.V. All rights reserved.
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