Journal
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
Volume 29, Issue 5, Pages 335-344Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/PAI.0000000000000866
Keywords
squamous cell carcinoma; mouth neoplasms; Hedgehog protein; epithelial-mesenchymal transition; cell proliferation
Funding
- National Council for Scientific and Technological Development (CNPq, Brazil)
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In oral squamous cell carcinoma, activation of the Hedgehog pathway may be associated with epithelial-mesenchymal transition and cell proliferation. Positive GLi-1 expression was correlated with loss of membrane E-cadherin and beta-catenin expression, while GLi-1 was also associated with clinical staging. The study suggests that the HH pathway plays a role in regulating the mesenchymal phenotype in OSCC.
In oral squamous cell carcinoma (OSCC), involvement and activation of the Hedgehog pathway (HH) may be related to epithelial-mesenchymal transition and cell proliferation. The present study aimed to evaluate epithelial-mesenchymal transition and proliferative potential in OSCC cases demonstrating activation of the HH pathway. Twenty-three GLi-1-positive OSCC cases were submitted to immunohistochemical detection of Snail, Slug, N-cadherin, E-cadherin, beta-catenin, and MCM3 proteins. Clinical-pathologic immunoexpression data were obtained from the invasion front and tumor islets, and then compared. At the invasion front, OSCC cases presented positive Snail, Slug, and MCM3 expression in the nuclei of tumor cells. Loss of membrane and cytoplasmic expression of E-cadherin and beta-catenin was also observed. Positive N-cadherin expression was observed in 31.78% of the cases. GLi-1 immunoexpression was associated with loss of membrane E-cadherin (P<0.001), membrane beta-catenin (P<0.001), and cytoplasmic beta-catenin (P=0.02) expression. In the tumor islets, we observed nuclear expression of GLi-1, Snail, Slug, and MCM3. E-cadherin and beta-catenin showed positivity in tumor cell membranes. Statistically significant positive correlations between GLi-1 and Snail (P=0.05), E-cadherin (P=0.01), and cytoplasmic beta-catenin (P=0.04) were found. GLi-1 was associated with clinical staging, while membrane beta-catenin expression was related to the presence of metastasis in lymph nodes and to clinical staging. The HH pathway may be involved in regulating the expression of the mesenchymal phenotype. The loss of membrane E-cadherin and beta-catenin expression was observed at the tumor front region, whereas cell adhesion protein expression was detected in tumor islets regardless of MCM3.
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