Journal
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
Volume 48, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.algal.2020.101891
Keywords
Antioxidant; Codium fragile; Sulfated polysaccharides; Vero cell; Zebrafish
Categories
Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2019R1A6A1A03033553]
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In the present study, the sulfated polysaccharides obtained from Codium fragile (CFCE-PS) were prepared by enzymatic digestion and ethanol precipitation, and the antioxidant properties of CFCE-PS were evaluated using both in vitro and in vivo models. CFCE-PS contained 76.84% sulfated polysaccharides, which comprised 55.78 +/- 3.00% of carbohydrate and 21.06 +/- 1.07% of sulfate. CFCE-PS scavenged hydroxyl, alkyl, and DPPH radicals with IC50 values of 1.21 +/- 0.01, 1.42 +/- 0.17, and 6.14 +/- 0.78 mg/mL, respectively. CFCE-PS remarkably protected Vero cells against hydrogen peroxide (H2O2)-induced damage by reducing the intracellular ROS level, increasing cell viability, and suppressing apoptosis in a dose-dependent manner. Furthermore, CFCE-PS significantly attenuated H2O2 -mediated oxidative stress in vivo in zebrafish. CFCE-PS dose-dependently improved the survival rate and normalized the heartbeat, as well as reduced ROS, cell death, and lipid peroxidation in H2O2-stimulated zebrafish. Taken together, these results demonstrated that CFCE-PS possesses strong in vitro and in vivo antioxidant activities, and it could be used as an ingredient in the functional food, pharmacy, and cosmetic industries.
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