Journal
TOXINS
Volume 12, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/toxins12070444
Keywords
fungal secondary metabolites; toxins; biological activity; biofilm; antibiotic-resistance; sphaeropsidin A; epi-epoformin
Categories
Funding
- UniNA
- Compagnia di San Paolo [E62F16001250003]
- DAAD Mobility Program 2018-2020
Ask authors/readers for more resources
Many pathogens involved in human infection have rapidly increased their antibiotic resistance, reducing the effectiveness of therapies in recent decades. Most of them can form biofilms and effective drugs are not available to treat these formations. Natural products could represent an efficient solution in discovering and developing new drugs to overcome antimicrobial resistance and treat biofilm-related infections. In this study, 20 secondary metabolites produced by pathogenic fungi of forest plants and belonging to diverse classes of naturally occurring compounds were evaluated for the first time against clinical isolates of antibiotic-resistant Gram-negative and Gram-positive bacteria.epi-Epoformin, sphaeropsidone, and sphaeropsidin A showed antimicrobial activity on all test strains. In particular, sphaeropsidin A was effective at low concentrations with Minimum Inhibitory Concentration (MIC) values ranging from 6.25 mu g/mL to 12.5 mu g/mL against all reference and clinical test strains. Furthermore, sphaeropsidin A at sub-inhibitory concentrations decreased methicillin-resistantS. aureus(MRSA) andP. aeruginosabiofilm formation, as quantified by crystal violet staining. Interestingly, mixtures of sphaeropsidin A andepi-epoformin have shown antimicrobial synergistic effects with a concomitant reduction of cytotoxicity against human immortalized keratinocytes. Our data show that sphaeropsidin A andepi-epoformin possess promising antimicrobial properties.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available