Journal
G3-GENES GENOMES GENETICS
Volume 10, Issue 9, Pages 2999-3008Publisher
GENETICS SOCIETY AMERICA
DOI: 10.1534/g3.120.401545
Keywords
Tumorigenesis; Neoplasia; Drosophila; EGFR; Hippo pathway
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Funding
- Indo-Danish research grant from Department of Biotechnology, Govt. of India
- Innovation fund Denmark
- Novo Nordisk Foundation [NNF12OC0000552]
- Neye Foundation
- Department of Science & Technology, Govt of India
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Genetic approaches inDrosophilahave successfully identified many genes involved in regulation of growth control as well as genetic interactions relevant to the initiation and progression of cancerin vivo. Here, we report on large-scale RNAi-based screens to identify potential tumor suppressor genes that interact with known cancer-drivers: the Epidermal Growth Factor Receptor and the Hippo pathway transcriptional cofactor Yorkie. These screens were designed to identify genes whose depletion drove tissue expressing EGFR or Yki from a state of benign overgrowth into neoplastic transformationin vivo. We also report on an independent screen aimed to identify genes whose depletion suppressed formation of neoplastic tumors in an existing EGFR-dependent neoplasia model. Many of the positives identified here are known to be functional in growth control pathways. We also find a number of novel connections to Yki and EGFR driven tissue growth, mostly unique to one of the two. Thus, resources provided here would be useful to all researchers who study negative regulators of growth during development and cancer in the context of activated EGFR and/or Yki and positive regulators of growth in the context of activated EGFR. Resources reported here are available freely for anyone to use.
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