Journal
ECOLOGY AND EVOLUTION
Volume 10, Issue 12, Pages 5219-5225Publisher
WILEY
DOI: 10.1002/ece3.5863
Keywords
conservation; European hare; mitochondrial DNA; molecular species identification
Categories
Funding
- FundacAo de Amparo a Pesquisa do Estado de SAo Paulo [FAPESP 2016/19106-1]
- CoordenacAo de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES) [001]
- FAPESP [2013/24453-4, 2018/11788-1]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [303101/2017-2, 308385/2014-4]
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Introduced in South America at the end of the 19th century, the European hare population has expanded dramatically and now represents a risk to native Brazilian forest rabbits. Monitoring the invasive Lepus europaeus and its coexistence with native Sylvilagus brasiliensis is a challenge that can be efficiently addressed by the use of molecular tools. This work describes a set of primers useful for amplifying three mini-barcodes for the molecular identification of both invasive and native leporid species using degraded fecal DNA. In addition, tests in silico indicate that these mini-barcodes can successfully amplify the DNA sequences of a number of leporids. These mini-barcodes constitute a powerful tool for the monitoring and management of the invasive L. europaeus and the conservation of native rabbits.
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