Journal
CELL REPORTS
Volume 32, Issue 6, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2020.108003
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Funding
- China Scholarships Council [201506230049]
- Sir Henry Dale Fellowship [102513/Z/13/Z]
- Division of Intramural Research, NHLBI/NIH
- NIH [R21HD084959, R35GM127075]
- Howard Hughes Medical Institute HHMI
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [ZIAHL006031] Funding Source: NIH RePORTER
- MRC [MR/R015635/1] Funding Source: UKRI
- Wellcome Trust [102513/Z/13/Z] Funding Source: Wellcome Trust
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Wnt3a-coated beads can induce asymmetric divisions of mouse embryonic stem cells (mESCs), resulting in one self-renewed mESC and one differentiating epiblast stem cell. This provides an opportunity for studying histone inheritance pattern at a single-cell resolution in cell culture. Here, we report that mESCs with Wnt3a-bead induction display nonoverlapping preexisting (old) versus newly synthesized (new) histone H3 patterns, but mESCs without Wnt3a beads have largely overlapping patterns, Furthermore, H4K20me2/3, an old histone-enriched modification, displays a higher instance of asymmetric distribution on chromatin fibers from Wnt3a-induced mESCs than those from non-induced mESCs. These locally distinct distributions between old and new histones have both cellular specificity in Wnt3a-induced mESCs and molecular specificity for histones H3 and H4. Given that post-translational modifications at H3 and H4 carry the major histone modifications, our findings provide a mammalian cell culture system to study histone inheritance for maintaining stem cell fate and for resetting it during differentiation.
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