Journal
SCIENTIFIC REPORTS
Volume 10, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41598-020-67163-7
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Funding
- Chang Gung Memorial Hospital grants [CMRPG3G0231, CMRPG3H1051, CMRPG3H1041]
- Ministry of Science and Technology Taiwan [MOST 106-2314-B-182A-131, MOST 107-2314-B-182A-101, MOST 106-2314-B-182A-034 -MY3]
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The effects of human amniotic fluid stem cell (hAFSC) transplantation on bladder function and molecular changes in spinal cord-injured (SCI) rats were investigated. Four groups were studied: sham and SCI plus phosphate-buffered saline (SCI+PBS), human embryonic kidney 293 (HEK293) cells, and hAFSCs transplantation. In SCI+PBS rat bladders, cystometry showed increased peak voiding pressure, voiding volume, bladder capacity, residual volume, and number of non-voiding contractions, and the total elastin/collagen amount was increased but collagen concentration was decreased at days 7 and 28. Immunoreactivity and mRNA levels of IGF-1, TGF-beta 1, and beta 3-adrenoceptor were increased at days 7 and/or 28. M2 immunoreactivity and M3 mRNA levels of muscarinic receptor were increased at day 7. M2 immunoreactivity was increased, but M2/M3 mRNA and M3 immunoreactivity levels were decreased at day 28. Brain derived-neurotrophic factor mRNA was increased, but immunoreactivity was decreased at day 7. HEK293 cell transplantation caused no difference compared to SCI+PBS group. hAFSCs co-localized with neural cell markers and expressed BDNF, TGF-beta 1, GFAP, and IL-6. The present results showed that SCI bladders released IGF-1 and TGF-beta 1 to stimulate elastin and collagen for bladder wall remodelling, and hAFSC transplantation improved these changes, which involved the mechanisms of BDNF, muscarinic receptors, and beta 3-adrenoceptor expression.
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