Journal
BIOMEDICAL OPTICS EXPRESS
Volume 11, Issue 8, Pages 4458-4470Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.398020
Keywords
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Funding
- Russian Science Foundation [19-15-00263]
- Russian Science Foundation [19-15-00263] Funding Source: Russian Science Foundation
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Although fluorescence lifetime imaging microscopy (FLIM) has been extensively applied to study cellular metabolism in the liver, there is neither an established approach to analyze the data, nor have appropriate protocols been developed to maintain the optical metabolic characteristics in the ex vivo liver tissue sample. Here, we show that a tri-exponential decay fitting model for the fluorescence signal from nicotinamide adenine dinucleotide (NAD(P)H) and the use of ex vivo samples allows the most appropriate processing of the FLIM data. Moreover, we determine the medium that maintains the initial metabolic state of hepatocytes (liver cells), most effectively. Our results should be particularly relevant for the interrogation of liver samples, not only in laboratory research, but also in clinical settings in the future. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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